Group 29 Presentation
Introduction
CD molecules are membrane proteins with diverse functions and distributions across immune cell types.
Their expression patterns help distinguish cell lineages and reveal functional relationships
The aim of our project was to answer the questions:
How do the expression of CD markers on lymphocyte subsets change during maturation?
How are fluorescence intensity, variability, and positivity (MedQb, CVQb, PEpos) related across CD markers in lymphocyte subsets?
Analysis 2
- Wide variations in CD marker distribution.
- Tissue-related clusters are common
- Some markers are universally expressed (e.g. CD45), while others are lineage-specific.
Analysis 4
Table: B cells had the most significant markers, showing stronger activation changes.
B cells: Tonsil B cells are more activated (CD69, CD80), while blood B cells mature gradually (CD11a, CD80).
CD4 T cells: Mature from thymus to blood, losing CD10 and gaining CD25 and CD4_MEM-241.
CD8 T cells: Move from active thymus cells to mature blood cells with higher CD27 and CD95.
Analysis 5
CD4 and CD8 T cells go through parallel stages
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How do the CD markers differ between CD4 and CD8 T cells for each stage?
- As expected CD4 and CD8 are significant different for all pairs
- CD59 is significant for all stages except TEMRA with a higher log(MedQb) for CD8
- SP1am and Naive have the lowest number of significant CDs → CD4 and CD8 more similar in these stages
