CD molecules are membrane proteins with diverse functions and distributions across immune cell types.
Their expression patterns help distinguish cell lineages and reveal functional relationships
The aim of our project was to answer the questions:
How do the expression of CD markers on lymphocyte subsets change during maturation?
How are fluorescence intensity, variability, and positivity (MedQb, CVQb, PEpos) related across CD markers in lymphocyte subsets?

Table: B cells had the most significant markers, showing stronger activation changes.
B cells: Tonsil B cells are more activated (CD69, CD80), while blood B cells mature gradually (CD11a, CD80).
CD4 T cells: Mature from thymus to blood, losing CD10 and gaining CD25 and CD4_MEM-241.
CD8 T cells: Move from active thymus cells to mature blood cells with higher CD27 and CD95.
CD4 and CD8 T cells go through parallel stages
How do the CD markers differ between CD4 and CD8 T cells for each stage?